Single-step and Multiple Bioassay Based on Combinable Pdms Capillary (cpc) Sensor Array

Combinable poly(dimethyl siloxane) (PDMS) capillary (CPC) sensor array allowing single step and multiple bioanalysis by simply introducing a sample solution by capillary action, followed by spontaneous mixing and reactions for fluorescence readout was developed. Since the CPC is fabricated by combining "Convex"-shaped PDMS plate immobilizing an insoluble membrane containing analytical reagents and "Concave"-shaped PDMS plate immobilizing a soluble membrane containing other kind of analytical reagents, it is very useful for designing various types of "single-step" bioassay. Here, enzyme inhibitor assay and glucose assay are presented as examples of single step assay. Furthermore, doping of PDMSpoly(ethyleneglycol) (PEG) copolymer into "concave"-shaped bulk PDMS gave fully hydrophilic surface, which solved the problems of capillary action-based sample introduction and homogeneous coating of highly hydrophilic PEG membrane on PDMS.